Variability within a pea core collection of LEAM and HSP22, two mitochondrial seed proteins involved in stress tolerance.
Identifieur interne : 002658 ( Main/Exploration ); précédent : 002657; suivant : 002659Variability within a pea core collection of LEAM and HSP22, two mitochondrial seed proteins involved in stress tolerance.
Auteurs : Marie-Hélène Avelange-Macherel [France] ; Nicole Payet [France] ; David Lalanne [France] ; Martine Neveu [France] ; Dimitri Tolleter [Australie] ; Judith Burstin [France] ; David Macherel [France]Source :
- Plant, cell & environment [ 1365-3040 ] ; 2015.
Descripteurs français
- KwdFr :
- Alignement de séquences, Graines (métabolisme), Génotype, Isoformes de protéines, Mitochondries (métabolisme), Pois (croissance et développement), Pois (génétique), Pois (physiologie), Protéines du choc thermique (génétique), Protéines du choc thermique (métabolisme), Protéines mitochondriales (génétique), Protéines mitochondriales (métabolisme), Protéines végétales (génétique), Protéines végétales (métabolisme), Stress physiologique, Séquence d'acides aminés, Température.
- MESH :
- croissance et développement : Pois.
- génétique : Pois, Protéines du choc thermique, Protéines mitochondriales, Protéines végétales.
- métabolisme : Graines, Mitochondries, Protéines du choc thermique, Protéines mitochondriales, Protéines végétales.
- physiologie : Pois.
- Alignement de séquences, Génotype, Isoformes de protéines, Stress physiologique, Séquence d'acides aminés, Température.
English descriptors
- KwdEn :
- Amino Acid Sequence, Genotype, Heat-Shock Proteins (genetics), Heat-Shock Proteins (metabolism), Mitochondria (metabolism), Mitochondrial Proteins (genetics), Mitochondrial Proteins (metabolism), Peas (genetics), Peas (growth & development), Peas (physiology), Plant Proteins (genetics), Plant Proteins (metabolism), Protein Isoforms, Seeds (metabolism), Sequence Alignment, Stress, Physiological, Temperature.
- MESH :
- chemical , genetics : Heat-Shock Proteins, Mitochondrial Proteins, Plant Proteins.
- chemical , metabolism : Heat-Shock Proteins, Mitochondrial Proteins, Plant Proteins.
- genetics : Peas.
- growth & development : Peas.
- metabolism : Mitochondria, Seeds.
- physiology : Peas.
- Amino Acid Sequence, Genotype, Protein Isoforms, Sequence Alignment, Stress, Physiological, Temperature.
Abstract
LEAM, a late embryogenesis abundant protein, and HSP22, a small heat shock protein, were shown to accumulate in the mitochondria during pea (Pisum sativum L.) seed development, where they are expected to contribute to desiccation tolerance. Here, their expression was examined in seeds of 89 pea genotypes by Western blot analysis. All genotypes expressed LEAM and HSP22 in similar amounts. In contrast with HSP22, LEAM displayed different isoforms according to apparent molecular mass. Each of the 89 genotypes harboured a single LEAM isoform. Genomic and RT-PCR analysis revealed four LEAM genes differing by a small variable indel in the coding region. These variations were consistent with the apparent molecular mass of each isoform. Indels, which occurred in repeated domains, did not alter the main properties of LEAM. Structural modelling indicated that the class A α-helix structure, which allows interactions with the mitochondrial inner membrane in the dry state, was preserved in all isoforms, suggesting functionality is maintained. The overall results point out the essential character of LEAM and HSP22 in pea seeds. LEAM variability is discussed in terms of pea breeding history as well as LEA gene evolution mechanisms.
DOI: 10.1111/pce.12480
PubMed: 25367071
Affiliations:
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(genetics)</term><term>Peas (growth & development)</term><term>Peas (physiology)</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (metabolism)</term><term>Protein Isoforms</term><term>Seeds (metabolism)</term><term>Sequence Alignment</term><term>Stress, Physiological</term><term>Temperature</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Alignement de séquences</term><term>Graines (métabolisme)</term><term>Génotype</term><term>Isoformes de protéines</term><term>Mitochondries (métabolisme)</term><term>Pois (croissance et développement)</term><term>Pois (génétique)</term><term>Pois (physiologie)</term><term>Protéines du choc thermique (génétique)</term><term>Protéines du choc thermique (métabolisme)</term><term>Protéines mitochondriales (génétique)</term><term>Protéines mitochondriales (métabolisme)</term><term>Protéines végétales (génétique)</term><term>Protéines végétales (métabolisme)</term><term>Stress physiologique</term><term>Séquence d'acides aminés</term><term>Température</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Heat-Shock Proteins</term><term>Mitochondrial Proteins</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Heat-Shock Proteins</term><term>Mitochondrial Proteins</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Pois</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Peas</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Peas</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Pois</term><term>Protéines du choc thermique</term><term>Protéines mitochondriales</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" qualifier="metabolism" 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xml:lang="en">LEAM, a late embryogenesis abundant protein, and HSP22, a small heat shock protein, were shown to accumulate in the mitochondria during pea (Pisum sativum L.) seed development, where they are expected to contribute to desiccation tolerance. Here, their expression was examined in seeds of 89 pea genotypes by Western blot analysis. All genotypes expressed LEAM and HSP22 in similar amounts. In contrast with HSP22, LEAM displayed different isoforms according to apparent molecular mass. Each of the 89 genotypes harboured a single LEAM isoform. Genomic and RT-PCR analysis revealed four LEAM genes differing by a small variable indel in the coding region. These variations were consistent with the apparent molecular mass of each isoform. Indels, which occurred in repeated domains, did not alter the main properties of LEAM. Structural modelling indicated that the class A α-helix structure, which allows interactions with the mitochondrial inner membrane in the dry state, was preserved in all isoforms, suggesting functionality is maintained. The overall results point out the essential character of LEAM and HSP22 in pea seeds. LEAM variability is discussed in terms of pea breeding history as well as LEA gene evolution mechanisms.</div></front></TEI><affiliations><list><country><li>Australie</li><li>France</li></country><region><li>Pays de la Loire</li></region><settlement><li>Angers</li></settlement><orgName><li>Université d'Angers</li></orgName></list><tree><country name="France"><region name="Pays de la Loire"><name sortKey="Avelange Macherel, Marie Helene" sort="Avelange Macherel, Marie Helene" uniqKey="Avelange Macherel M" first="Marie-Hélène" last="Avelange-Macherel">Marie-Hélène Avelange-Macherel</name></region><name sortKey="Burstin, Judith" sort="Burstin, Judith" uniqKey="Burstin J" first="Judith" last="Burstin">Judith Burstin</name><name sortKey="Lalanne, David" sort="Lalanne, David" uniqKey="Lalanne D" first="David" last="Lalanne">David Lalanne</name><name sortKey="Macherel, David" sort="Macherel, David" uniqKey="Macherel D" first="David" last="Macherel">David Macherel</name><name sortKey="Neveu, Martine" sort="Neveu, Martine" uniqKey="Neveu M" first="Martine" last="Neveu">Martine Neveu</name><name sortKey="Payet, Nicole" sort="Payet, Nicole" uniqKey="Payet N" first="Nicole" last="Payet">Nicole Payet</name></country><country name="Australie"><noRegion><name sortKey="Tolleter, Dimitri" sort="Tolleter, Dimitri" uniqKey="Tolleter D" first="Dimitri" last="Tolleter">Dimitri Tolleter</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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